The Full Human Immune System Mouse Models: in vivo pharmacology fee-for-services

TransCure bioServices has developed a strong expertise, data set driven for the 100% functional reconstitution of a full human immune system in severely immunodeficient mouse strains (NOG, BRG, BRG-S, NCG) via engraftment of human umbilical cord blood CD34+ stem cells.

Full Human Immune System Reconstitution

Engraftment and differentiation of human stem cells

High And Stable Humanization Rate

Flow Cytometry Characterization

Human Cells in Peripheral Blood of hu-Mice

Human Cell type Expressed In HIS Marker
Leucocytes CD45
T-Lymphocytes CD3, CD4, CD8, CD25
B-Lymphocytes CD19
NK cells CD16, CD56
Dendritic cells CD1c, CD303,CD141, CD11c
Monocytes CD14
Macrophages CD11b
Activated T and/or NK cells HLA-DR, CD69

Human Cytokines in hu-Mice

Cytokine Expressed In HIS Method of Detection
huTNF-α IHC
IFN- FC, RT-PCR
IL-2 FC
IL-4 FC
IL-17 FC, RT-PCR
IL-23 RT-PCR

In details, following chemoablation, the young animals are engrafted with human CD34+ Hematopoietic Stem Cells (HSC) isolated from human cord blood in our BSL-2 animal facility. 14 weeks post-CD34+ engraftment, the rate of the full human immune system reconstitution is assessed and the human immune cell population is monitored by state-of-the-art flow cytometry.

Preparation of the Hu-mouse models

Isolation of the human CD34+ stem cells from cord blood

  • Source: EFS (Etablissement Français du Sang) Besançon, France
  • Ethic: Informed/signed consent by the parents and anonymized
  • Transport: within 48h after delivery
  • Volume : +/- 100ml
  • Number of cells: 1-2x109
  • % of CD34 : 0.3-0.7%

Find out about our fee-for-services: Cancer/Immuno-oncology/PDX, HIV, Inflammation/IBD,Vaccine/Immunization (coming soon)

 


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