Core technology
Flow cytometry Platform
The AttuneTM NxT Flow Cytometer combines precision with performance in a true benchtop flow cytometer with 3 different type of detections: Forward-scattered light (FSC), Side-scattered light (SSC) and Photomultiplier tubes (PMTs).
- Empower your research — Up to 10x faster than traditional flow cytometric analyzers
- Enable more — Analyze more sample types, including tumor samples, without losing samples
- Expand your capabilities — Enable more as your needs expand, with field upgradability of up to 4 lasers and 16 detection channels
- Simplified sample prep — No-wash, no-lyse sample prep options with clog-resistant fluidics
- Option for walk-away automation — Run difficult samples with a system that is less sensitive to clogging
- Flexible — Up to 14 colors and 6 channels off the violet laser
Immuno-profiling and TILs
Complete Tumor and Tissue Analysis
- Mechanical and enzymatic digestion of the sampels using Gentle MACS Octo Dissociator
- Flow cytometry analysis of tumor antigens and human immune cells infiltration
IHC
Histology Platform
Services
- Paraffin inclusion
- Paraffin/Cryosection
- H&E staining
- Histopathology (colon)
- Immunohistology (Chromogenic and Fluorescence)
- Digitalization and image Quantification
PDX studies
Patient-Derived Xenograft (PDX) in hu-mouse
Partnership with Champions Oncology: TransCure becomesthe european hub and operational center- Large cohort (>1’000 patient tumors) of TumorGraft®
- Multiple tumor types
- Fully characterized and searchable (medical history, genetic, mutations, growth, in vivo drug responses, …)
- Faithful reconstitution of patient tumor biology in hu-mouse models
QRT-PCR
Content QRT-PCR
Under construction
In-vivo imaging
Small Animal in vivo Imaging
We can monitor, in a living animal, orthotopic, metastatic dissemination and subcutaneous tumor progression.
The NEWTON bioluminescence imaging system allows for the non-invasive detection and quantification from the onset throughout full development of any tumor.
Applications:
• tumor growth monitoring
• orthotopic mouse models
• metastatic dissemination
Luciferase tumor cell line available:
• human: PC-3M, LnCaP, MDA-MB-231,
A549, HCT 116, U87-MG, …
• mouse: 4T1, B16F10, …
• Autobioluminescence
In-vitro assays
- Humoral response (Ab secretion, B cell characterisation)
- Cell mediated immune response (T cell characterization, INFg, granzymeB, Perforin (intra-cellular staining and secretion)
- Cytokine quantification in peripheral blood and tissues (CBA, ELISA)
- Ligand Binding assay (receptor occupancy assay)
- PBMC, Splenocytes, bone marrow immuno-phenotype : intra-cellular and membrane staining
- In vitro ADCC assays (Phagocytosis & cytotoxicity)
- In vitro cell culture assays (MTS, apoptosis, necrosis, release of immunogenic materials)
- Elisa/Elispot
HUMAN IMMUNE SYSTEM hu-mouse models
